- "Disguise and subterfuge are their meat and drink. They are assassins, spies of the highest order. No one knows who they are. No one has ever seen their faces and lived. They come to us masked if they mean us no harm. Masked in the manner of Venice when the rich and powerful wore masks, caps, and hats, so all looked alike, and none could be distinguished from the other. If they appear before us in those costumes, then they are merely here to observe. If they appear in the masks of their namesakes, then it could go either way. They could be merely observing, or they could mean to kill us. They would wear their namesakes, both to hide their faces and to let us know that if we do not cooperate they could turn deadly."
The Harlequin or The Nameless Vampire Guards are the closest thing to police that vampires have in the Anita Blake: Vampire Hunter series. They wear white masks or masks of their namesakes to conceal their identities; moreover, they also use masks to send messages and officially contact a master of the city.
They were created by the Mother of All Darkness to serve her and the vampire council. After the Mother's defeat, there is a division among the members; some join Jean-Claude and Anita Blake, while others try to defeat them.
In the process of Anita recovering parts of Marmee Noir's power, more and more Harlequin recognizes her and Jean-Claude.
The Harlequin are modeled in style on the Commedia dell'arte. There are four masks that they send to a master of the city as declaration of intentions and official sign of contact:
- White - Observation
- Red - Pain, punishment
- Black - Death
- White with little gold musical notes - They wish to meet, not torment or kill, but to talk
They are based on the myths of the wild hunt and were established to control the vampires. It is composed of very old and powerful vampires who are capable of not just manipulating the behaviors and emotions of humans or younger vampires and lycanthropes, but even master vampires, or alpha weres. They owe allegiance to no one line, can fly and punish the wicked and kill mysteriously and quickly.
Their names are: Harlequin, Punchinello, Scaramouche, Pierrot/Pierrette, Columbine, Hanswurst, Il Dottore.
Once a member dies, a new vampire assumes the name and mask; if impossible, their name and mask are supposed to be retired .
Mentioning the name Harlequin without being contacted by them first is cause for death sentence, until Jean-Claude inherits them after Marmee Noir's death and changes the rule.
After the Mother of all Darkness encouraged the First Emperor of China Qin Shi Huang to kill the last High Queen of the Yellow Clan, she permitted the Harlequin to keep their yellow tigers as their animals to call, but continued to hunt the Yellow Clan to extinction. Secretly some of the Harlequin spared some of the yellow tigers and hid them from Marmee Noir, breeding the bloodline until the day they put the Darkness back to sleep.
One thousand years ago the Harlequin used a spell, a sacrifice of lives and the power of the yellow tigers to bind Marmee Noir to her latest inhabited body and to put her to sleep. Although they intended to kill her, they couldn't figure out how to do that.
After Marmee Noir was put to sleep the Harlequin continued to do their job, but some started to work as assassins for members of the Vampire Council. When Marmee Noir started waking up from her sleep, Pantalone, Columbine and other rogue Harlequin came to St. Louis with the intention of taking it over. They were stopped and killed by Anita, Jean-Claude and their people.
After the body of the Mother of all Darkness was destroyed, and she possessed some of the Council members, a civil war started between the members of the Harlequin that still served her and those that wanted her dead. (Bullet)
Introduced in The Harlequin:
- Jake - Werewolf that protects Anita when she is mentally attacked by the Harlequin. He reveals himself to be one of them and that he came to investigate the activities of the rogue Harlequin. He later assists Anita with the golden weretigers.
- Pantalone (former Pantaleon) - Leader of the rogue Harlequin. He, Nivia and Mercia took assassination jobs from council members. Fearing the return of Marmee Noir he tries to kill Jean-Claude, Anita and Richard and become the master of the city of St. Louis. He was both vampire and werewolf (and ancient wolf) and could control the darkness and hide inside it. (Deceased)
- Columbine - Mercia before she was recruited by Pantalone. She tries to "steal" the congregation of the Church of Eternal Life. She could create and feed on doubt. (Deceased)
- Giovanni - The human servant of Columbine. (Deceased)
- Nivia ("civilian" name before she was recruited by Pantalone) - A vampire that tried to turn Anita, Jean-Claude and Richard against each other. (Deceased)
- Soledad - A golden weretiger, she was Nivia's animal to call. (Deceased)
Introduced in Skin Trade:
- Sebastian - A black weretiger, he was Vittorio's tiger to call, and after his master lost his powers became a Harlequin.
Introduced in Affliction:
- Mischa - A vampire, master of Goran. He followed Jean-Claude after Anita killed the dark mother. His name with the Harlequin has been Graziano.
- Goran - A werebear and the animal to call of Mischa.
- Jane - A vampire and the master of Seamus.
- Seamus - A werehyena of African origin and the animal to call of Jane. Nicknamed "Dark Water" by some of the guards.
- Nilda (Brunhilda) - a werebear from an extinct species of red bear. Was being abused by her Master Gunnar. Develops a relationship with Ethan.
Introduced in Hit List:
- George - A red weretiger one of the Harlequin that kidnapped Anita in Hit List. (Deceased)
- Marius - Nicknamed Harley by Anita. A wereleopard and one of the Harlequins that kidnapped Anita in Hit List. (Deceased)
- Thaddeus - A werewolf and one of the Harlequin that kidnapped Anita in Hit List. Sacrificed himself to save Lisandro. (Deceased)
- Unnamed vampire - Master of George. Beheaded while he slept so George would die. (Deceased)
- Unnamed vampire - Master of Marius. Beheaded while he slept so Marius would die. (Deceased)
- Unnamed vampire - Master of unnamed wereleopard. Beheaded while he slept so his animal to call would die. (Deceased)
- Unnamed shapeshifter - Burned alive. (Deceased)
- Unnamed wereleopard - One of the Harlequin that kidnapped Anita in Hit List. Died after her master was beheaded. (Deceased)
- Thing one - A werelion male and one of the Harlequin that kidnapped Anita in Hit List.
- Thing two - A werelion female and one of the Harlequin that kidnapped Anita in Hit List.
Introduced in Bullet:
- Yiyú aka Jade - A black weretiger, she was abused by her master for centuries. Anita breaks her bond with her master and marks Jade as her own black tiger to call.
- Jade's master - A black vampire, and the former master of Jade. He sent Jade to spy on Anita and Jean-Claude during their meeting with the weretigers.
- Topaz - A golden weretiger who comes to St. Louis to see if Anita and Jean-Claude could become Master's of all Tigers.
Introduced in Dead Ice:
- Magda - A werelion, her master is Giacomo.
- Fortune - A blue weretiger, Echo's animal to call.
- Echo - A vampire, Fortune's master.
Introduced in Affliction:
- Brunhilda aka Nilda - A werebear, her master is Gunnar.
- Gunnar - Nilda’s master, abusive. (Mentioned only)
Introduced in Crimson Death:
- Giacomo - Vampire, master of Magda.
- Kaazim - A werejackal who helped Jake hide the golden tigers.
- Bilquees (Queenie?) - A vampire, Kaazim's master.
- Scaramouche - A wererat.
- Pierette - A wereleopard.
- Pierrot - Pierrette's master.
- Hortensio - A wereanimal.
- Magnifico - Hortensio's master.
- Rodrigo - Wereleopard, triplet.
- Rodina - Wereleopard, triplet.
- Ru - Wereleopard, triplet.